[Product Name] Generic Name: Mycoplasma Antibody Rapid Test Card (Colloidal Gold Method)
[Packaging Specification] 50T/box
[Principle] The Mycoplasma antibody rapid detection card is made using the colloidal gold immunochromatography test (GICA) principle. After the sample is added to the sample well and moves along the chromatographic membrane together with the colloidal gold label, it binds to the colloidal gold marker and antigen on the detection line, resulting in a purplish-red color. If there is no mycoplasma antibody, no color reaction occurs.
[Kit Composition]
Serial number | Name | 50 per box |
1. | Test card (including straw) | 50 units |
2. | Sample diluent | 50 copies |
[Storage and Shelf Life] Store in a cool, dry place (2-30°C), shelf life is 24 months.
[Sample Requirements]
1. The Mycoplasma antibody rapid test card is only suitable for detecting serum/whole blood from pigs, cattle, and sheep.
2. Serum: Draw 2-3ml of blood by conventional method and place it in a clean, dry test tube, let it stand for about 1 hour until coagulated, then centrifuge at 4000 rpm for 10 minutes (blood can also be left to stand for about 2 hours until coagulation and naturally precipitates), then separate the serum. The serum must be clear, without hemolysis or contamination. Serum samples can be stored at 2~8°C in the short term, and at -20°C in the long term.
3. Whole blood: Use fresh anticoagulant whole blood on the same day; untreated whole blood is used freshly collected.
[Test Method]
1. Serum/whole blood sample addition method: Tear open the aluminum foil packaging of the test card, take out the test card, place it on a flat, clean counter, use the pipette inside the bag to slowly drop 1 drop of serum or 2 drops of whole blood into the filling well, then add 2 drops of diluent to the filling well.
2. Leave at room temperature for 10-15 minutes to determine the result; color results after 15 minutes are invalid.
[Result Judgment]
1. Negative: In the observation hole, only a purplish-red line appears in the reference line area (C).
2. Positive: In the observation well, both the detection line area (T) and the control line area (C) show purple-red lines. The higher the antibody titer, the darker the color of the test line (T).
3. Ineffective: No purplish-red lines appear in the reference line area (C) inside the observation hole.
[Diagnostic Reference]
1. If the pig, cattle, or sheep have not been vaccinated against mycoplasma:
A. When no obvious color bands appear at the test line T of the sample, the sample does not have
Contains mycoplasma antibodies; if the animal is healthy, it indicates no mycoplasma infection.
B. When a clear color band appears at the test line T of the sample, the animal is present
In cases of mycoplasma infection or previous infection, further verification is required.
2. If the pig, cattle, or sheep have been vaccinated against mycoplasma:
A. When no obvious color bands appear at the test line T of the sample, it indicates vaccine immunity
No protective antibodies have been produced, there is a risk of infection with wild toxins, and vaccination is recommended for supplementary vaccination.
B. When a clear color band appears at the test line T of the sample, it indicates that the vaccine has produced protective antibodies, and the likelihood of being infected with the wild toxin is low. But as expected
If symptoms of mycoplasma infection appear, it cannot be ruled out that the infection is caused by immune failure.
[Limitations of the Test Method]
This test card is for screening only and does not serve as a basis for diagnosis.
[Notes]
1. Please read the manual carefully before testing. All reagents provided by this product are for use only.
2. Do not use other animal serum or other liquids that are not required for the sample as negative controls.
3. Products that are expired or have damaged aluminum foil bags must not be used.
4. When removing the Mycoplasma antibody rapid test card from the refrigerator, it should be returned to room temperature before opening. Once opened, the test card should be used as soon as possible to avoid deactivation after moisture.
5. Avoid using contaminated, cloudy, severely hemolytic, or large blood lipid samples.
6. Avoid contact with the chromatography film at the sample holes and detection windows on the test card.
7. Waste from the test should be considered pollutants and properly handled according to local regulations.
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